Microenvironnement and Therapeutic Resistance
in Pancreatic Neoplasms


Pancreatic ductal adenocarcinoma (PDAC), commonly known as pancreatic cancer, is a very aggressive tumor since it presents a rapid metastatic potential and a strong therapeutic resistance. This tumor has the particularity of being rich in stroma (up to 80% of its tumor volume), composed of a very fibrotic extracellular matrix, and of immune and non-immune stromal cells, of which cancer-associated fibroblasts (CAFs) are the majority. My research team is specifically interested in the role of CAFs in the aggressiveness of pancreatic cancer cells, with the objective of understanding the biology of CAFs, their dialogues with cancer cells and with other stromal cells (endothelial and immune), and how these dialogues impact the resistance of cancer cells to chemotherapies or immunotherapies. The understanding of these inter-cellular dialogues and with the extracellular matrix allows us to propose and test anti-cancer therapies co-targeting cancer cells and their stroma, in particular by targeted nanotherapies approaches (Veronique Gigoux’s project).

Thus, to understand the tumor-stroma dialogues correlated with the aggressiveness of pancreatic cancer, our team is i/ developing models integrating this stroma and mimicking the pathology in the patient, such as PDX (Patient-Derived Xenografts) and primocultures of CAFs and tumor cells grown in 3 dimensions (tumor organoids) and in co-culture with CAFs, as well as murine pancreatic cancer models (genetically modified mice developing spontaneous cancer, and orthotopic surgical transplantation of pancreatic cancer cells with CAFs) ; and ii/ performings separate analyses of gene expression in the tumor epithelial compartment and in the stromal compartment, using transcriptomics (mRNA expression), translactomics (translated RNA expression, project of Yvan Martineau), and matrisome (exhaustive analysis of matrix proteins expression, Christine Jean’s project) on PDX (hybrid grafted tumor models) naïve to treatment or rendered chemoresistant (Corinne Bousquet’s project), and/or by bioinformatics deconvolution on bulk RNAseq of human tumors.

Tumor organoid (left: immunofluorescence to visualize the cytokeratin cytoskeleton in green, the actin filaments particularly abundant at the brush border in pink and the nuclei in blue; right: transmission electron microscopy).

Pancreatic cancer

Microenvironment & Cancer-Associated Fibroblasts



Translational regulation

Metabolic reprogramming

Matrix rigidity & mechano-transduction

Patient-derived experimental models

Targeted nanotherapies

(polysome) RNAseq & bioinformatics


Multiplexed tissue imaging



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team members

Séverine Garnier
Ingénieur ou technicien / Engineer or Technician
Mehdi Liauzun
Etudiant ou stagiaire / PhD student or Trainee
Marjorie Fanjul
Enseignant-chercheur / Teacher-researcher
Jacobo Solorzano
Etudiant ou stagiaire / PhD student or Trainee
Veronique Gigoux
Chercheur / Researcher
Ahmed Abdelhamid
Etudiant ou stagiaire / PhD student or Trainee
Hippolyte Audureau
Ingénieur ou technicien / Engineer or Technician
Justine Journaux
Etudiant ou stagiaire / PhD student or Trainee
Loubna Laib
Chercheur / Researcher
Corinne Bousquet
Chercheur / Researcher
Yvan Martineau
Chercheur / Researcher
Emilie Decaup
Ingénieur ou technicien / Engineer or Technician
Alexia Brunel
Etudiant ou stagiaire / PhD student or Trainee
Claire Lac
Etudiant ou stagiaire / PhD student or Trainee
Christine Jean
Chercheur / Researcher
Ismahane Belhabib
Etudiant ou stagiaire / PhD student or Trainee

parternships & FUNDING

Centre de Recherches en Cancérologie de Toulouse

Centre de Recherches en Cancérologie de Toulouse (Oncopole)

Toulouse – FR

Nous contacter

05 82 74 15 75

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