EQUIPMENT
– geNomics

  & TRANSCRIPTOMIcs

Chromium Single Cell Controller

 

Single cell isolation for gene expression analysis in NGS sequencing (10X Genomics)

This technology allows the separation of 500 to 10,000 cells/sample and the analysis of their individual gene expression. The approach to cellular heterogeneity is made possible. The principle is based on the encapsulation of a cell in an oil droplet containing a bead on which poly dT oligonucleotides are attached to capture mRNAs. Uniqueness is given by the use of a specific barcode for each bead.

In order to better identify subpopulations or to follow the expression of a surface protein of interest, it is possible to couple the measurement of individual gene expression with the identification of cell surface proteins by the CITE-Seq method (antibody labelling).

Other applications are gradually being developed: VDJ, ATAC-Seq (epigenetics), CNV, etc.

 

Access to this technology is by project service and specific quote.

Centre de Recherches en Cancérologie de Toulouse

Centre de Recherches en Cancérologie de Toulouse (Oncopole)

Toulouse – FR

Nous contacter

05 82 74 15 75

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