EQUIPMENT
– geNomics
& TRANSCRIPTOMIcs
Chromium Single Cell Controller
Single cell isolation for gene expression analysis in NGS sequencing (10X Genomics)
This technology allows the separation of 500 to 10,000 cells/sample and the analysis of their individual gene expression. The approach to cellular heterogeneity is made possible. The principle is based on the encapsulation of a cell in an oil droplet containing a bead on which poly dT oligonucleotides are attached to capture mRNAs. Uniqueness is given by the use of a specific barcode for each bead.
In order to better identify subpopulations or to follow the expression of a surface protein of interest, it is possible to couple the measurement of individual gene expression with the identification of cell surface proteins by the CITE-Seq method (antibody labelling).
Other applications are gradually being developed: VDJ, ATAC-Seq (epigenetics), CNV, etc.
Access to this technology is by project service and specific quote.
Toulouse Cancer Research Center (Oncopole)
Toulouse - FR
CRCT-Oncopole Follow 1,433 1,279
Compte officiel du Centre de Recherches en Cancérologie de Toulouse
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🇬🇧Cytidine deaminase-dependent mitochondrial biogenesis as a potential vulnerability in pancreatic cancer cells. ImPact team @CordelierPierre article from @crctoncopole published in @CommsBio
➡️https://www.crct-inserm.fr/en/targeting-the-energy-production-process-in-pancreatic-cancer-cells-which-relies-on-an-enzyme-called-cytidine-deaminase-could-make-these-cells-more-vulnerable/
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