Équipe
SALVATORE VALITUTTI

DynAct :

Dynamique moléculaire des interactions lymphocytaires

 

L’équipe fait partie du consortium « ATTACK Cancer  » financé par un ERC Synergy Grant, visant à mettre au point des traitements anticancéreux plus efficaces et plus ciblés.

Le laboratoire d’Excellence Toulouse Cancer est un projet qui vise à comprendre les mécanismes de résistance et de rechute dans les cancers.

Les spécificités
de notre axe de recherche

Les réponses immunitaires sont basées sur des interactions multiples et dynamiques entre les cellules du système immunitaire conduisant à la formation de zones de signalisation spécialisées, les synapses immunologiques.

Notre équipe applique une combinaison d’approches de pointe, incluant l’imagerie des cellules vivantes rapide et la microscopie de super-résolution (sur des cellules isolées et des tranches de tissus humains) mais également la modélisation mathématique, de manière à visualiser et interpréter les dynamiques moléculaires se produisant au niveau des synapses immunologiques. Décrypter la communication intercellulaire au niveau des synapses immunologiques peut contribuer à concevoir des stratégies thérapeutiques innovantes visant à moduler les réponses immunitaires dans différents contextes pathologiques.

Synapse immunologique

  Lymphocytes T cytotoxiques

Immunologie tumorale
Techniques d’imagerie en temps réel

Immuno-évasion tumorale

Pyroptose

Biologie computationnelle

Modélisation in silico
Vision par ordinateur

Programmation génétique cartésienne

T CD8+ mémoire-résidents

     Microenvironement tumoral

DES PROJETS
DE RECHERCHE

LES FOCUS
DE L’ÉQUIPE

PRODUCTIONS SCIENTIFIQUES

PUBLICATIONS 2024
Zevolini, Fabrizia, Anna Onnis, Roxana Khazen, Sabina Müller, Giuseppe Marotta, Salvatore Valitutti, Francesca Finetti, and Cosima T. Baldari. “Polo-like Kinase 1 Regulates Immune Synapse Assembly and Cytotoxic T Cell Function by Driving Microtubule Dynamics.” Journal of Cell Science 137, no. 5 (March 1, 2024): jcs261476. https://doi.org/10.1242/jcs.261476.
PUBLICATIONS 2023
Baldari, Cosima T., Salvatore Valitutti, and Michael L. Dustin. “Editorial: Mechanisms of Lymphocyte Mediated Cytotoxicity in Health and Disease.” Frontiers in Immunology 14 (2023): 1226870. https://doi.org/10.3389/fimmu.2023.1226870.
Müller, Sabina, Liza Filali, Marie-Pierre Puissegur, and Salvatore Valitutti. “Measuring CTL Lytic Granule Secretion and Target Cell Membrane Repair by Fluorescent Lipophilic Dye Uptake at the Lytic Synapse.” Methods in Molecular Biology (Clifton, N.J.) 2654 (2023): 463–76. https://doi.org/10.1007/978-1-0716-3135-5_30.
PUBLICATIONS 2022
McKenzie, Brienne, and Salvatore Valitutti. “Resisting T Cell Attack: Tumor-Cell-Intrinsic Defense and Reparation Mechanisms.” Trends in Cancer, December 31, 2022, S2405-8033(22)00264-3. https://doi.org/10.1016/j.trecan.2022.12.003.
Puissant-Lubrano, Bénédicte, Charlène Bouthemy, Nicolas Congy-Jolivet, Jean Milhes, Vincent Minville, Nassim Kamar, Leïla Demini, Franck Zal, and Yves Renaudineau. “The Oxygen Carrier M101 Alleviates Complement Activation, Which May Be Beneficial for Donor Organ Preservation.” Frontiers in Immunology 13 (December 9, 2022): 1006761. https://doi.org/10.3389/fimmu.2022.1006761.
Cassioli, Chiara, Laura Patrussi, Salvatore Valitutti, and Cosima T. Baldari. “Learning from TCR Signaling and Immunological Synapse Assembly to Build New Chimeric Antigen Receptors (CARs).” International Journal of Molecular Sciences 23, no. 22 (November 17, 2022): 14255. https://doi.org/10.3390/ijms232214255.
Leveque, Edouard, Axel Rouch, Charlotte Syrykh, Julien Mazières, Laurent Brouchet, Salvatore Valitutti, Eric Espinosa, and Fanny Lafouresse. “Phenotypic and Histological Distribution Analysis Identify Mast Cell Heterogeneity in Non-Small Cell Lung Cancer.” Cancers 14, no. 6 (March 9, 2022): 1394. https://doi.org/10.3390/cancers14061394.
Filali, Liza, Marie-Pierre Puissegur, Kevin Cortacero, Sylvain Cussat-Blanc, Roxana Khazen, Nathalie Van Acker, François-Xavier Frenois, et al. “Ultrarapid Lytic Granule Release from CTLs Activates Ca2+-Dependent Synaptic Resistance Pathways in Melanoma Cells.” Science Advances 8, no. 7 (February 18, 2022): eabk3234. https://doi.org/10.1126/sciadv.abk3234.
Renaudineau, Yves, Laurent Sailler, Florence Abravanel, Jacques Izopet, Adrien Delourme, Damien Biotti, Jonathan Ciron, et al. “Glucocorticoid Use as a Cause of Non-Cellular Immune Response to SARS-Cov2 Spike in Patients with Immune System Diseases.” Journal of Autoimmunity 133 (2022): 102912. https://doi.org/10.1016/j.jaut.2022.102912.
McKenzie, Brienne, Roxana Khazen, and Salvatore Valitutti. “Greek Fire, Poison Arrows, and Scorpion Bombs: How Tumor Cells Defend Against the Siege Weapons of Cytotoxic T Lymphocytes.” Frontiers in Immunology 13 (2022): 894306. https://doi.org/10.3389/fimmu.2022.894306.
PUBLICATIONS 2021
Laplagne, Chloé, Laetitia Ligat, Juliet Foote, Frederic Lopez, Jean-Jacques Fournié, Camille Laurent, Salvatore Valitutti, and Mary Poupot. “Self-Activation of Vγ9Vδ2 T Cells by Exogenous Phosphoantigens Involves TCR and Butyrophilins.” Cellular & Molecular Immunology, June 28, 2021. https://doi.org/10.1038/s41423-021-00720-w.
Lafouresse, Fanny, Romain Jugele, Sabina Müller, Marine Doineau, Valérie Duplan-Eche, Eric Espinosa, Marie-Pierre Puisségur, Sébastien Gadat, and Salvatore Valitutti. “Stochastic Asymmetric Repartition of Lytic Machinery in Dividing CD8+ T Cells Generates Heterogeneous Killing Behavior.” ELife 10 (January 11, 2021): e62691. https://doi.org/10.7554/eLife.62691.
PUBLICATIONS 2020
Bálint, š., S. Müller, R. Fischer, B. M. Kessler, M. Harkiolaki, S. Valitutti, and M. L. Dustin. “Supramolecular Attack Particles Are Autonomous Killing Entities Released from Cytotoxic T Cells.” Science 368, no. 6493 (May 22, 2020): 897–901. https://doi.org/10.1126/science.aay9207.
Biolato, Andrea Michela, Liza Filali, Hannah Wurzer, Céline Hoffmann, Ernesto Gargiulo, Salvatore Valitutti, and Clément Thomas. “Actin Remodeling and Vesicular Trafficking at the Tumor Cell Side of the Immunological Synapse Direct Evasion from Cytotoxic Lymphocytes.” In International Review of Cell and Molecular Biology, 356:99–130. Elsevier, 2020. https://doi.org/10.1016/bs.ircmb.2020.07.001.
PUBLICATIONS 2019
Khazen, Roxana, Sabina Müller, Fanny Lafouresse, Salvatore Valitutti, and Sylvain Cussat-Blanc. “Sequential Adjustment of Cytotoxic T Lymphocyte Densities Improves Efficacy in Controlling Tumor Growth.” Scientific Reports 9, no. 1 (August 23, 2019): 12308. https://doi.org/10.1038/s41598-019-48711-2.
Cordella, Martina, Claudio Tabolacci, Cinzia Senatore, Stefania Rossi, Sabina Mueller, Carla Lintas, Adriana Eramo, et al. “Theophylline Induces Differentiation and Modulates Cytoskeleton Dynamics and Cytokines Secretion in Human Melanoma-Initiating Cells.” Life Sciences 230 (August 1, 2019): 121–31. https://doi.org/10.1016/j.lfs.2019.05.050.
Gonnord, Pauline, Manon Costa, Arnaud Abreu, Michael Peres, Loïc Ysebaert, Sébastien Gadat, and Salvatore Valitutti. “Multiparametric Analysis of CD8+ T Cell Compartment Phenotype in Chronic Lymphocytic Leukemia Reveals a Signature Associated with Progression toward Therapy.” Oncoimmunology 8, no. 4 (2019): e1570774. https://doi.org/10.1080/2162402X.2019.1570774.

LES MEMBRES DE L’ÉQUIPE

Amandine Michelet
Doctorant / PhD student
Mara Cenerenti
Doctorant / PhD student
Roxana Khazen
Chercheur Post-Doctorant / Post-Doc researcher
Emma Cini
Ingénieur de laboratoire / laboratory engineer
Zevolini Fabrizia
Doctorant / PhD student
Kévin Cortacero
Doctorant / PhD student
Salvatore Valitutti
Chercheur statutaire / permanent scientist
Chloé Lamesa
Clinicien Chercheur / Researcher hospital practitioner
Cécile Demeur
Enseignant chercheur / University researcher
Sabina Mueller
Ingénieur de laboratoire / laboratory engineer
Marie-Pierre Puisségur
Ingénieur de laboratoire / laboratory engineer
Brienne McKenzie
Chercheur Post-Doctorant / Post-Doc researcher
Lucie Demeersseman
Ingénieur de laboratoire / laboratory engineer
Gaëlle Corsaut
Ingénieur de laboratoire / laboratory engineer
Frecia Rodriguez
Chercheur invité / visiting researcher
Fanny Lafouresse
Chercheur statutaire / permanent scientist

LES PARTENAIRES & FINANCIERS

Centre de Recherches en Cancérologie de Toulouse

Centre de Recherches contre le Cancer de Toulouse (Oncopole)

Toulouse - FR

Nous contacter

+33 5 82 74 15 75

Nous rejoindre ?

Centre de Recherches en Cancérologie de Toulouse

Centre de Recherches en Cancérologie de Toulouse (Oncopole)

Toulouse – FR

Nous contacter

05 82 74 15 75

Envie de rejoindre
L’équipe du CRCT ?

Pin It on Pinterest