Conférence – Matrix regulation by LYVE-1+ tissue macrophages – Véronique Angeli · C3M Nice

Matrix regulation by LYVE-1⁺ tissue macrophages

Macrophages are integral component of the tissue and not only immune cells in charge of defense against pathogens. They are essential in tissue development, homeostasis and diseases. We identified a subset of tissue resident macrophages that expresses LYVE-1 and is conserved across tissues and species. In mice, we showed that LYVE-1⁺ macrophages are specialized in regulating the composition and organization of the extracellular matrix in different tissues. Through this regulatory mechanisms, LYVE-1⁺ macrophages control the biomechanical properties of the tissues and influence the responses to different stimuli. However, isolation of primary human tissue macrophages is challenging and limits functional investigations on human LYVE-1+ macrophages. Thus, we developed a method to generate LYVE-1⁺ macrophages from human monocytes that phenotypically, transcriptionally and functionally resemble the in vivo counterparts. With this unique approach, we integrated human LYVE-1-like macrophages to 2D tissue matrix or tumoroids to study their behavior using live imaging. Our preliminary results and future directions will be discussed.

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