{"id":44130,"date":"2021-12-16T16:29:19","date_gmt":"2021-12-16T15:29:19","guid":{"rendered":"https:\/\/www.crct-inserm.fr\/?page_id=44130"},"modified":"2024-01-08T14:52:18","modified_gmt":"2024-01-08T13:52:18","slug":"dynact-en","status":"publish","type":"page","link":"https:\/\/www.crct-inserm.fr\/en\/dynact-en\/","title":{"rendered":"DynAct"},"content":{"rendered":"

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TEAM
SALVATORE VALITUTTI<\/h1>\n

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DynAct :
Molecular dynamics of Lymphocyte Interactions<\/b><\/h2>\n

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 <\/p>\n

The team is part of the ERC Synergy Grant funded “ATTACK Cancer” consortium, aiming to develop more effective and targeted cancer treatments.<\/o:p><\/span><\/p>\n

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The Toulouse Cancer Laboratory of Excellence is a project that aims to understand the mechanisms of resistance and relapse in cancer.<\/span><\/o:p><\/span><\/b><\/p>\n

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THE SPECIFITIES
<\/b>OUR RESEARCH AXIS<\/h2>\n

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<\/o:p><\/span><\/b><\/p>\n

Immune responses are based on multiple and dynamic interactions between cells of the immune system leading to the formation of specialized signaling areas, the immunological synapses. <\/span><\/p>\n

Our team applies a combination of state-of-the-art approaches, including fast live cell imaging and super-resolution microscopy (on single cells and human tissue slices) as well as mathematical modeling, in order to visualize and interpret the molecular dynamics occurring at immunological synapses. Deciphering the intercellular communication at the level of immunological synapses may contribute to the design of innovative therapeutic strategies aimed at modulating immune responses in different pathological contexts. Our research develops along the following 4 main axes.<\/span><\/o:p><\/span><\/b><\/p>\n

 <\/p>\n

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Immunological synapse<\/p>\n

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Cytotoxic T lymphocytes<\/o:p><\/span><\/p>\n

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Tumor immunology<\/o:p><\/span><\/p>\n

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Real-time imaging techniques<\/o:p><\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_4,1_4,1_4,1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Open Sans|700|||||||” text_orientation=”center” custom_margin=”||15px||false|false” custom_padding=”15px||15px||false|false” border_color_bottom=”#F19532″ border_width_left=”5px” border_color_left=”#F19532″ global_colors_info=”{}”]<\/p>\n

Tumor immunoevasion<\/o:p><\/span><\/p>\n

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Pyroptosis<\/o:p><\/span><\/p>\n

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Computational biology<\/o:p><\/span><\/p>\n

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In silico modeling<\/o:p><\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_4,1_4,1_4,1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Open Sans|700|||||||” text_orientation=”center” custom_margin=”||15px||false|false” custom_padding=”15px||15px||false|false” border_color_bottom=”#F19532″ border_width_left=”5px” border_color_left=”#F19532″ global_colors_info=”{}”]<\/p>\n

Computer vision<\/o:p><\/span><\/p>\n

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Cartesian genetic programming<\/o:p><\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Open Sans|700|||||||” text_orientation=”center” custom_margin=”||15px||false|false” custom_padding=”15px||15px||false|false” border_color_bottom=”#F19532″ border_width_left=”5px” border_color_left=”#F19532″ global_colors_info=”{}”]<\/p>\n

CD8+ memory-resident
<\/span> T cells<\/o:p><\/span><\/p>\n

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\u00a0 \u00a0 \u00a0Tumor microenvironment<\/p>\n

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RESEARCH PROJECTS<\/b><\/h2>\n

[\/et_pb_text][et_pb_divider _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.16″ _module_preset=”default” custom_padding=”||0px|||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_blog fullwidth=”off” posts_number=”5″ include_categories=”252″ use_manual_excerpt=”off” show_more=”on” show_author=”off” show_date=”off” show_categories=”off” masonry_tile_background_color=”#f2efef” _builder_version=”4.16″ _module_preset=”default” header_level=”h3″ header_font=”|800|||||||” header_font_size=”20px” body_font_size=”20px” read_more_text_color=”#5EC7ED” read_more_font_size=”20px” read_more_line_height=”3em” background_enable_color=”off” border_width_all=”0px” global_colors_info=”{}”][\/et_pb_blog][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”off|off|off” _builder_version=”4.16″ _module_preset=”default” custom_padding=”||0px|||” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”448e09ae-9652-4ead-bc5f-152c7adf1202″ global_colors_info=”{}”]<\/p>\n

THE TEAM’S
<\/strong>FOCUS<\/h2>\n

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Discover<\/h4>\n

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Understand<\/h4>\n

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Participate<\/h4>\n

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\n VALITUTTI LAB<\/span>\n <\/span>\n Suivre<\/span>\n \n <\/path><\/svg>5<\/span>\n <\/path><\/svg>30<\/span>\n <\/span>\n <\/p>\n

\n We study molecular dynamics of lymphocyte interactions using high end imaging technics. #ImmunologicalSynapse #CTL #cancer #immunotherpay #modeling <\/p>\n <\/div>\n

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The \"most wanted\" beautiful review of our great \"cell-death mechanims\" expert, Dr. Brienne Mckenzie, on how target cell resist CTL attack , is now available for everyone to read. Enjoy your read \ud83d\ude42 and spread the word!
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Zevolini, Fabrizia, Anna Onnis, Roxana Khazen, Sabina Müller, Giuseppe Marotta, Salvatore Valitutti, Francesca Finetti, and Cosima T. Baldari. “Polo-like Kinase 1 Regulates Immune Synapse Assembly and Cytotoxic T Cell Function by Driving Microtubule Dynamics.” Journal of Cell Science<\/i> 137, no. 5 (March 1, 2024): jcs261476. https:\/\/doi.org\/10.1242\/jcs.261476<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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Baldari, Cosima T., Salvatore Valitutti, and Michael L. Dustin. “Editorial: Mechanisms of Lymphocyte Mediated Cytotoxicity in Health and Disease.” Frontiers in Immunology<\/i> 14 (2023): 1226870. https:\/\/doi.org\/10.3389\/fimmu.2023.1226870<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\t\t\t\t
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Müller, Sabina, Liza Filali, Marie-Pierre Puissegur, and Salvatore Valitutti. “Measuring CTL Lytic Granule Secretion and Target Cell Membrane Repair by Fluorescent Lipophilic Dye Uptake at the Lytic Synapse.” Methods in Molecular Biology (Clifton, N.J.)<\/i> 2654 (2023): 463–76. https:\/\/doi.org\/10.1007\/978-1-0716-3135-5_30<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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McKenzie, Brienne, Roxana Khazen, and Salvatore Valitutti. “Greek Fire, Poison Arrows, and Scorpion Bombs: How Tumor Cells Defend Against the Siege Weapons of Cytotoxic T Lymphocytes.” Frontiers in Immunology<\/i> 13 (2022): 894306. https:\/\/doi.org\/10.3389\/fimmu.2022.894306<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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Laplagne, Chloé, Laetitia Ligat, Juliet Foote, Frederic Lopez, Jean-Jacques Fournié, Camille Laurent, Salvatore Valitutti, and Mary Poupot. “Self-Activation of Vγ9Vδ2 T Cells by Exogenous Phosphoantigens Involves TCR and Butyrophilins.” Cellular & Molecular Immunology<\/i>, June 28, 2021. https:\/\/doi.org\/10.1038\/s41423-021-00720-w<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\t\t\t\t
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Lafouresse, Fanny, Romain Jugele, Sabina Müller, Marine Doineau, Valérie Duplan-Eche, Eric Espinosa, Marie-Pierre Puisségur, Sébastien Gadat, and Salvatore Valitutti. “Stochastic Asymmetric Repartition of Lytic Machinery in Dividing CD8+ T Cells Generates Heterogeneous Killing Behavior.” ELife<\/i> 10 (January 11, 2021): e62691. https:\/\/doi.org\/10.7554\/eLife.62691<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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Khazen, Roxana, Sabina Müller, Fanny Lafouresse, Salvatore Valitutti, and Sylvain Cussat-Blanc. “Sequential Adjustment of Cytotoxic T Lymphocyte Densities Improves Efficacy in Controlling Tumor Growth.” Scientific Reports<\/i> 9, no. 1 (August 23, 2019): 12308. https:\/\/doi.org\/10.1038\/s41598-019-48711-2<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\t\t\t\t
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Cordella, Martina, Claudio Tabolacci, Cinzia Senatore, Stefania Rossi, Sabina Mueller, Carla Lintas, Adriana Eramo, et al. “Theophylline Induces Differentiation and Modulates Cytoskeleton Dynamics and Cytokines Secretion in Human Melanoma-Initiating Cells.” Life Sciences<\/i> 230 (August 1, 2019): 121–31. https:\/\/doi.org\/10.1016\/j.lfs.2019.05.050<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\t\t\t\t
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Gonnord, Pauline, Manon Costa, Arnaud Abreu, Michael Peres, Loïc Ysebaert, Sébastien Gadat, and Salvatore Valitutti. “Multiparametric Analysis of CD8+ T Cell Compartment Phenotype in Chronic Lymphocytic Leukemia Reveals a Signature Associated with Progression toward Therapy.” Oncoimmunology<\/i> 8, no. 4 (2019): e1570774. https:\/\/doi.org\/10.1080\/2162402X.2019.1570774<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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TEAM MEMBERS<\/strong><\/h2>\n

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Amandine Michelet<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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Mara Cenerenti<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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Roxana Khazen<\/a><\/div>\n
Chercheur Post-Doctorant \/ Post-Doc researcher<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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Emma Cini<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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Zevolini Fabrizia<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
K\u00e9vin Cortacero<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Salvatore Valitutti<\/a><\/div>\n
Chercheur statutaire \/ permanent scientist<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Chlo\u00e9 Lamesa<\/a><\/div>\n
Clinicien Chercheur \/ Researcher hospital practitioner<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\n
\"\"<\/a><\/div>\n
C\u00e9cile Demeur<\/a><\/div>\n
Enseignant chercheur \/ University researcher<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
\n
\n
\"\"<\/a><\/div>\n
Sabina Mueller<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\n
\"\"<\/a><\/div>\n
Marie-Pierre Puiss\u00e9gur<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\n
\"\"<\/a><\/div>\n
Brienne McKenzie<\/a><\/div>\n
Chercheur Post-Doctorant \/ Post-Doc researcher<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
\n
\n
\"\"<\/a><\/div>\n
Lucie Demeersseman<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
\n
\n
\"\"<\/a><\/div>\n
Ga\u00eblle Corsaut<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\n
\"\"<\/a><\/div>\n
Frecia Rodriguez<\/a><\/div>\n
Chercheur invit\u00e9 \/ visiting researcher<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Fanny Lafouresse<\/a><\/div>\n
Chercheur statutaire \/ permanent scientist<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n <\/div>\n<\/div><\/div>\n\n
<\/div><\/div>\n\n