{"id":43295,"date":"2021-12-06T13:16:17","date_gmt":"2021-12-06T12:16:17","guid":{"rendered":"https:\/\/www.crct-inserm.fr\/?page_id=43295"},"modified":"2024-01-08T14:44:32","modified_gmt":"2024-01-08T13:44:32","slug":"sigdyn_en","status":"publish","type":"page","link":"https:\/\/www.crct-inserm.fr\/en\/sigdyn_en\/","title":{"rendered":"SigDYN_en"},"content":{"rendered":"

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Team
Julie Guillermet-Guibert<\/h1>\n

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SigDYN : <\/strong><\/h2>\n

Integrated cellular signalling and PI3K isoforms<\/strong><\/h2>\n

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the specifities<\/strong><\/h2>\n

of our research axis<\/h2>\n<\/div>\n<\/div>\n

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The team directed by J Guillermet-Guibert studies the family of lipid kinases known as phosphoinositide 3-kinases (PI3Ks) from different angles. This family of enzymes is involved in major cellular functions such as cell survival, proliferation, growth, migration, differentiation but also protein synthesis and intracellular vesicular trafficking. In vertebrates, the PI3K family is divided into three different classes. This classification is based on their structure, mode of activation and lipid substrate specificity in vitro and in vivo. These enzymes, encoded by 8 different genes, all phosphorylate the hydroxyl group in position 3 of the inositol ring of phosphoinositides, hence their name.<\/p>\n

Only class I (composed of PI3K\u03b1, PI3K\u03b2, PI3K\u03b3, PI3K\u03b4) can convert phosphatidylinositol (4,5)-bisphosphate [PtdIns-4,5-P2, PtdIns (4,5) P2, PIP2] to phosphatidylinositol (3,4,5)-trisphosphate [PtdIns-3,4,5-P3, PtdIns (3,4,5) P3, PIP3]. The substrate of class I enzymes is mainly located at the cell membrane. Thus, activation of class I PI3Ks produces a lipid second messenger at the plasma membrane at the interface with the cytoplasm, which allows the transmission of biochemical information into the cytoplasm and leads to a cellular response.<\/p>\n

Class II (PI3KC2\u03b1, PI3KC2\u03b2, PI3KC2\u03b3) and class III (VPS34) PI3Ks can generate phosphatidylinositol 3-phosphate [PtdIns-3-P, PtdIns (3) P, PI-3-P]. Class II PI3Ks can also synthesise phosphatidylinositol (3,4)-bisphosphate [PtdIns-3, 4-P2, PtdIns (3,4) P2]. The substrates of these enzymes are present in the outer membranes of organelles within the cell, at the level of endosomes, autophagosomes. Thus, class II and III PI3Ks control intracellular vesicular trafficking.<\/p>\n

The high activation of class I PI3Ks is considered to be a characteristic of cancer; however, the role of each class I PI3K in the different stages of carcinogenesis is poorly understood. This research is carried out by our team.<\/p>\n

The roles of class II and III PI3Ks in cancer are poorly studied. We have undertaken to understand them.<\/p>\n

We mostly apply our research to solid cancers such as pancreatic cancer to better understand, earlier diagnose and treat this deadly disease. We also work on ovarian cancers and collaborate with teams working on liquid cancers.<\/p>\n

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Oncogenic signalling<\/p>\n

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PI3K<\/p>\n

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Targeted therapies<\/p>\n

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r\u00e9sistance<\/p>\n

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Tumour niche<\/p>\n

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Cancer initiation<\/p>\n

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Mechanobiology<\/p>\n

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compression<\/p>\n

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Genetically modified mice<\/p>\n

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Tumour imaging<\/p>\n

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Pancreatic cancer<\/p>\n

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Ovarian cancer<\/p>\n

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RESEARCH <\/strong>PROJECTS<\/strong><\/h2>\n

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THE TEAM’S
<\/strong>FOCUS<\/h2>\n

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Discover<\/h4>\n

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Understand<\/h4>\n

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Participate<\/h4>\n

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En live<\/strong><\/h2>\n

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\n Guillermet's Lab<\/span>\n <\/span>\n Suivre<\/span>\n \n <\/path><\/svg>60<\/span>\n <\/path><\/svg>73<\/span>\n <\/span>\n <\/p>\n

\n Julie Guillermet-Guibert's lab located in https:\/\/t.co\/Y4Z7QkJ9AU, working on signaling dynamics and focusing on PI3K in pancreatic cancer. <\/p>\n <\/div>\n

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\n <\/path><\/svg> <\/div>\n \"GuillermetLab\"\n <\/div>\n <\/a>\n<\/div>\n\n
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\n \n \"GuillermetLab;\n <\/a>\n \n Guillermet's Lab<\/a>\n @GuillermetLab<\/a>\n \n \t\t ·<\/span>\n\t
\n 13 Sep 1701994422034915368<\/span><\/a>\n <\/div>\n <\/div>\n
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And now Maria, @CayronCayron, @SanzharAitbay and @BenoitThibault_ are presenting their posters! <\/p>\n\t\t\t\t\t\t\n \n

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\"And<\/div>\n <\/div>\n\n \n
\n Guillermet's Lab<\/span>\n @GuillermetLab<\/span>\n

A part of the team (@CayronCayron @SanzharAitbay @BenoitThibault_ Julie and Maria) has the great chance to attend to the Biochemical Society congress on PI3K\/Akt\/mTOR in basic research and clinical translation in Barcelona.
\n#BiochemicalSociety #PI3K <\/p>\n <\/div>\n<\/a>\n \n <\/div>\n \n\t\n

\n \n <\/path><\/g><\/svg> R\u00e9pondre sur Twitter 1701994422034915368<\/span>\n <\/a>\n\n \n <\/path><\/svg> Retweeter sur Twitter 1701994422034915368<\/span>\n 0<\/span>\n <\/a>\n\n \n <\/path><\/g><\/svg> J\u2019aime sur Twitter 1701994422034915368<\/span>\n 8<\/span>\n <\/a>\n\n \n Twitter<\/span>\n 1701994422034915368<\/span>\n <\/a>\n \n\t<\/div>\n\n\t<\/div>\n\n <\/div>\n\n Voir plus<\/span><\/a>\n\n<\/span>\n<\/div>\n[\/et_pb_code][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” background_color=”#f2efef” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”448e09ae-9652-4ead-bc5f-152c7adf1202″ global_colors_info=”{}”]<\/p>\n

SCIENTIFIC PRODUCTIONS<\/strong><\/strong><\/h2>\n

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\n\n\t\t7813506<\/span>\n\t\t<\/span>\n\t\tPKLPPQDF<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t2024<\/span>\n <\/span>\n items<\/span>\n\t\t1<\/span>\n\t\tchicago-fullnote-bibliography<\/span>\n\t\t0<\/span>\n\t\tdate<\/span>\n\t\tdesc<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t<\/span>\n\t\t<\/span>\n <\/span>\n 43295<\/span>\n\t\thttps:\/\/www.crct-inserm.fr\/wp-content\/plugins\/zotpress\/<\/span>\n\n\t\t
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%20western%20blot.%20Results%3A%20We%20demonstrate%20that%20both%20nuclear%20and%20membrane%20ER%5Cu03b1%20actions%20are%20required%20for%20the%20pro-tumoral%20effects%20of%20E2%2C%20while%20tamoxifen%20totally%20abrogates%20the%20E2-induced%20in%20vivo%20tumor%20growth%2C%20through%20inhibition%20of%20angiogenesis%20but%20promotion%20of%20vessel%20normalization.%20RNA%20sequencing%20indicates%20that%20tamoxifen%20inhibits%20the%20E2-induced%20genes%2C%20but%20also%20initiates%20a%20specific%20transcriptional%20program%20that%20especially%20regulates%20angiogenic%20genes%20and%20differentially%20regulates%20glycolysis%2C%20oxidative%20phosphorylation%20and%20inflammatory%20responses%20in%20endothelial%20cells.%20Conclusion%3A%20These%20findings%20provide%20evidence%20that%20tamoxifen%20specifically%20inhibits%20angiogenesis%20through%20a%20reprogramming%20of%20endothelial%20gene%20expression%20via%20regulation%20of%20some%20transcription%20factors%2C%20that%20could%20open%20new%20promising%20strategies%20to%20manage%20cancer%20therapies%20affecting%20the%20tumor%20microenvironment%20of%20ER%5Cu03b1-negative%20tumors.%22%2C%22date%22%3A%222024%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.7150%5C%2Fthno.87306%22%2C%22ISSN%22%3A%221838-7640%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22PKLPPQDF%22%5D%2C%22dateModified%22%3A%222024-01-08T13%3A30%3A37Z%22%7D%7D%5D%7D<\/span>\n\n\t\t\t\t
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Fébrissy, Chanaëlle, Marine Adlanmerini, Christel Péqueux, Frédéric Boudou, Mélissa Buscato, Adrien Gargaros, Silveric Gilardi-Bresson, et al. “Reprogramming of Endothelial Gene Expression by Tamoxifen Inhibits Angiogenesis and ERα-Negative Tumor Growth.” Theranostics<\/i> 14, no. 1 (2024): 249–64. https:\/\/doi.org\/10.7150\/thno.87306<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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Chaves-Almagro, Carline, Johanna Auriau, Alizée Dortignac, Pascal Clerc, Hubert Lulka, Simon Deleruyelle, Fabrice Projetti, et al. “Upregulated Apelin Signaling in Pancreatic Cancer Activates Oncogenic Signaling Pathways to Promote Tumor Development.” International Journal of Molecular Sciences<\/i> 23, no. 18 (September 13, 2022): 10600. https:\/\/doi.org\/10.3390\/ijms231810600<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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Mazloumi Gavgani, Fatemeh, Thomas Karlsson, Ingvild L. Tangen, Andrea Papdiné Morovicz, Victoria Smith Arnesen, Diana C. Turcu, Sandra Ninzima, et al. “Nuclear Upregulation of Class I Phosphoinositide 3-Kinase P110β Correlates with High 47S RRNA Levels in Cancer Cells.” Journal of Cell Science<\/i> 134, no. 3 (February 10, 2021). https:\/\/doi.org\/10.1242\/jcs.246090<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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Rizzuti, Ilaria Francesca, Pietro Mascheroni, Silvia Arcucci, Zacchari Ben-Mériem, Audrey Prunet, Catherine Barentin, Charlotte Rivière, et al. “Mechanical Control of Cell Proliferation Increases Resistance to Chemotherapeutic Agents.” Physical Review Letters<\/i> 125, no. 12 (September 18, 2020): 128103. https:\/\/doi.org\/10.1103\/PhysRevLett.125.128103<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\t\t\t\t
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ll%20as%20in%20their%20volume.%20Increase%20in%20rigidity%20in%20tumors%20detected%20by%20ultrafast%20elasticity%20imaging%20coincided%20with%20detection%20of%20tumors%20by%20echography%20and%20with%20the%20detection%20of%20the%20inflammatory%20protumoral%20systemic%20condition%20by%20non%20invasive%20follow-up%20and%20of%20collagen%20fibers%20by%20post-processing%20tumoral%20IHC%20analysis.%20Conclusion%3A%20Our%20promising%20results%20indicate%20the%20potential%20of%20the%20shear%20wave%20elastography%20to%20support%20individualization%20of%20diagnosis%20in%20this%20most%20aggressive%20disease.%22%2C%22date%22%3A%222019%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.7150%5C%2Fthno.34066%22%2C%22ISSN%22%3A%221838-7640%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22PKLPPQDF%22%5D%2C%22dateModified%22%3A%222021-04-26T15%3A23%3A38Z%22%7D%7D%5D%7D<\/span>\n\n\t\t\t\t
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Zamora, Audrey, Melinda Alves, Charlotte Chollet, Nicole Therville, Tiffany Fougeray, Florence Tatin, Camille Franchet, et al. “Paclitaxel Induces Lymphatic Endothelial Cells Autophagy to Promote Metastasis.” Cell Death & Disease<\/i> 10, no. 12 (December 20, 2019): 956. https:\/\/doi.org\/10.1038\/s41419-019-2181-1<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\t\t\t\t
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Müller, David, Sauyeun Shin, Théo Goullet de Rugy, Rémi Samain, Romain Baer, Manon Strehaiano, Laia Masvidal-Sanz, et al. “EIF4A Inhibition Circumvents Uncontrolled DNA Replication Mediated by 4E-BP1 Loss in Pancreatic Cancer.” JCI Insight<\/i> 4, no. 21 (November 1, 2019). https:\/\/doi.org\/10.1172\/jci.insight.121951<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\t\t\t\t
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Therville, Nicole, Silvia Arcucci, Aurélie Vertut, Fernanda Ramos-Delgado, Dina Ferreira Da Mota, Marlène Dufresne, Céline Basset, and Julie Guillermet-Guibert. “Experimental Pancreatic Cancer Develops in Soft Pancreas: Novel Leads for an Individualized Diagnosis by Ultrafast Elasticity Imaging.” Theranostics<\/i> 9, no. 22 (2019): 6369–79. https:\/\/doi.org\/10.7150\/thno.34066<\/a>.<\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n\n\n

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team members
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\"\"<\/a><\/div>\n
Corentin Bouvier<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Silvia Arcucci<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Romina D'ANGELO<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Sanzhar Aitbay<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Maria Chaouki<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Julien Faccini<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Hala Shalhoub<\/a><\/div>\n
Chercheur Post-Doctorant \/ Post-Doc researcher<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Julie Guillermet-Guibert<\/a><\/div>\n
Chercheur statutaire \/ permanent scientist<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Nicole Therville<\/a><\/div>\n
Ing\u00e9nieur de laboratoire \/ laboratory engineer<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Beno\u00eet Thibault<\/a><\/div>\n
Chercheur statutaire \/ permanent scientist<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Mickael Di-Luoffo<\/a><\/div>\n
Chercheur Post-Doctorant \/ Post-Doc researcher<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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Camille Guyon<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n
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\"\"<\/a><\/div>\n
Coralie Cayron<\/a><\/div>\n
Doctorant \/ PhD student<\/div><\/div>\n<\/div><\/div><\/div> <\/div>\n <\/div>\n<\/div><\/div>\n\n
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